SHC1 antibody [3A1]

Cat. No. GTX83656

Host	Mouse
Clonality	Monoclonal
Clone Name	3A1
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human
Package	100 μl ($519)

See all SHC1 products

Datasheet
Document

View product citations for antibody GTX83656 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
ICC/IF	1:100
IHC-P	1:50
IP	Assay dependent

Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SHC1 (NP_003020) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SHC adaptor protein 1 , SHC , SHCA

Cellular Localization

Cytoplasm

Background

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span.

Database

Gene ID: 6464 SHC1
UniProt: P29353 SHC1

Research Area

DATA IMAGES

GTX83656 IHC-P Image

IHC-P analysis of bladder tissue using GTX83656 SHC1 antibody [3A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83656 IHC-P Image

IHC-P analysis of colon tissue using GTX83656 SHC1 antibody [3A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83656 IHC-P Image

IHC-P analysis of liver carcinoma tissue using GTX83656 SHC1 antibody [3A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83656 WB Image

WB analysis of HEK293T cells transfected with SHC1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83656 SHC1 antibody [3A1].
Loading : 5 ug per lane

GTX83656 IHC-P Image

IHC-P analysis of lung tissue using GTX83656 SHC1 antibody [3A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83656 IHC-P Image

IHC-P analysis of prostate carcinoma tissue using GTX83656 SHC1 antibody [3A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83656 IHC-P Image

IHC-P analysis of breast tissue using GTX83656 SHC1 antibody [3A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83656 IHC-P Image

IHC-P analysis of prostate tissue using GTX83656 SHC1 antibody [3A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83656 IHC-P Image

IHC-P analysis of kidney tissue using GTX83656 SHC1 antibody [3A1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83656 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SHC1 plasmid using GTX83656 SHC1 antibody [3A1].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

SHC1 antibody [3A1]

Cat. No. GTX83656

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX83656 IHC-P Image

GTX83656 IHC-P Image

GTX83656 IHC-P Image

GTX83656 WB Image

GTX83656 IHC-P Image

GTX83656 IHC-P Image

GTX83656 IHC-P Image

GTX83656 IHC-P Image

GTX83656 IHC-P Image

GTX83656 ICC/IF Image

REFERENCE

REVIEW

Product

Research

Support

Company

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