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SHMT2 antibody

KO/KD
Anti-SHMT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64542
Anti-SHMT2 antibody used in Immunoprecipitation (IP). GTX64542
Anti-SHMT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64542
Anti-SHMT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64542
Anti-SHMT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64542
Anti-SHMT2 antibody used in Western Blot (WB). GTX64542
Anti-SHMT2 antibody used in Western Blot (WB). GTX64542
Anti-SHMT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64542

Cat. No. GTX64542

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SHMT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine hydroxymethyltransferase 2 , GLYA , HEL-S-51e , SHMT

Cellular Localization

Mitochondrion,Mitochondrion inner membrane,Cytoplasm,Nucleus

Background

This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-SHMT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64542

GTX64542 ICC/IF Image

ICC/IF analysis of L929 cells using GTX64542 SHMT2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-SHMT2 antibody used in Immunoprecipitation (IP). GTX64542

GTX64542 IP Image

IP analysis of HeLa cell lysate using GTX64542 SHMT2 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-SHMT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64542

GTX64542 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX64542 SHMT2 antibody.
Dilution : 1:100

Anti-SHMT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64542

GTX64542 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX64542 SHMT2 antibody.
Dilution : 1:100

Anti-SHMT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64542

GTX64542 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64542 SHMT2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-SHMT2 antibody used in Western Blot (WB). GTX64542

GTX64542 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX64542 SHMT2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SHMT2 antibody used in Western Blot (WB). GTX64542

GTX64542 WB Image

WB analysis of various sample lysates using GTX64542 SHMT2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SHMT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64542

GTX64542 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX64542 SHMT2 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399