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SIRT1 antibody [GT1189]

Recombinant antibody KO/KD Orthogonal
Anti-SIRT1 antibody [GT1189] used in Western Blot (WB). GTX00951
Anti-SIRT1 antibody [GT1189] used in Western Blot (WB). GTX00951
Anti-SIRT1 antibody [GT1189] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00951
Anti-SIRT1 antibody [GT1189] used in Western Blot (WB). GTX00951

Cat. No. GTX00951

Reference 2

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1189

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all SIRT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human SIRT1.

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2885208

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sirtuin 1 , SIR2 , SIR2L1 , SIR2alpha , SIRT1 , SIR1

Cellular Localization

Nucleus,PML body,Cytoplasm,Mitochondrion

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

Database

Research Area

DATA IMAGES

Anti-SIRT1 antibody [GT1189] used in Western Blot (WB). GTX00951

GTX00951 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SIRT1 antibody [GT1189] (GTX00951) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SIRT1 antibody [GT1189] used in Western Blot (WB). GTX00951

GTX00951 WB Image

WB analysis of various samples using GTX00951 SIRT1 antibody [GT1189].
Dilution : 1:1000
Loading : 25μg

Anti-SIRT1 antibody [GT1189] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00951

GTX00951 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX00951 SIRT1 antibody [GT1189].
Red : Primary antibody
Blue : DAPI

Anti-SIRT1 antibody [GT1189] used in Western Blot (WB). GTX00951

GTX00951 WB Image

KO/KD

WB analysis of wild-type (WT) and SIRT1 knockout (KO) HeLa cell extracts using GTX00951 SIRT1 antibody [GT1189].
Dilution : 1:1000
Laoding : 25μg

REFERENCE

Application Reference

Guo Z et al. Bioengineered 2021; 12 (2) : 11030-11040 Zafirlukast ameliorates Docetaxel-induced activation of NOD-like receptor protein 3 (NLRP3) inflammasome, mediated by sirtuin1 (SIRT1) in hepatocytes.
Application : WB
Reactivity : Human
Hsu WH et al. Aging (Albany NY) 2020; 12 (11) : 10556-10577 T1-11, an adenosine derivative, ameliorates aging-related behavioral physiology and senescence markers in aging mice.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399