GeneTex

  • Country / Location Selection

United States (US)

SKA2 antibody

Anti-SKA2 antibody used in Western Blot (WB). GTX85247

Cat. No. GTX85247

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all SKA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

3T3 (NIH) Cell Lysate(GTX27179)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SKA2 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human SKA2.The immunogen is located within the first 50 amino acids of SKA2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

spindle and kinetochore associated complex subunit 2 , FAM33A

Background

Upon entry into mitosis, the cell’s microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing. SKA2 has also been shown to interact with glucocorticoid receptors and to be involved in glucocorticoid signaling and cell proliferation.

Database

Research Area

DATA IMAGES

Anti-SKA2 antibody used in Western Blot (WB). GTX85247

GTX85247 WB Image

WB analysis of 3T3 cell lysate using GTX85247 SKA2 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

REFERENCE

There are currently no references for SKA2 antibody (GTX85247). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for SKA2 antibody (GTX85247). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399