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SMC2 antibody

Anti-SMC2 antibody used in Western Blot (WB). GTX130078
Anti-SMC2 antibody used in Immunoprecipitation (IP). GTX130078

Cat. No. GTX130078

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SMC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886170

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

structural maintenance of chromosomes 2 , CAP-E , CAPE , SMC-2 , SMC2L1

Cellular Localization

Nucleus , Cytoplasm

Background

Members of the structural maintenance of chromosomes, or SMC, family (e.g., SMC1A; MIM 300040) are critical for mitotic chromosome condensation in frogs and for DNA repair in mammals.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SMC2 antibody used in Western Blot (WB). GTX130078

GTX130078 WB Image

SMC2 antibody detects SMC2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC2 antibody (GTX130078) diluted at a dilution of 1:1000.

Anti-SMC2 antibody used in Immunoprecipitation (IP). GTX130078

GTX130078 IP Image

Immunoprecipitation of SMC2 protein from HeLa whole cell extracts using 5 μg of SMC2 antibody (GTX130078).
Western blot analysis was performed using SMC2 antibody (GTX130078).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169