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SMCR8 antibody

Anti-SMCR8 antibody used in Western Blot (WB). GTX134785
Anti-SMCR8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134785
Anti-SMCR8 antibody used in IHC (Paraffin sections) (IHC-P). GTX134785
Anti-SMCR8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134785

Cat. No. GTX134785

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all SMCR8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMCR8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887344

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMCR8-C9orf72 complex subunit

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-SMCR8 antibody used in Western Blot (WB). GTX134785

GTX134785 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMCR8 antibody (GTX134785) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMCR8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134785

GTX134785 ICC/IF Image

SMCR8 antibody detects SMCR8 protein at nucleus by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMCR8 stained by SMCR8 antibody (GTX134785) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.

Anti-SMCR8 antibody used in IHC (Paraffin sections) (IHC-P). GTX134785

GTX134785 IHC-P Image

SMCR8 antibody detects SMCR8 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SMCR8 stained by SMCR8 antibody (GTX134785) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SMCR8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134785

GTX134785 ICC/IF Image

SMCR8 antibody detects SMCR8 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMCR8 stained by SMCR8 antibody (GTX134785) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169