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SNRPA antibody

Anti-SNRPA antibody used in IHC (Paraffin sections) (IHC-P). GTX101664
Anti-SNRPA antibody used in IHC (Paraffin sections) (IHC-P). GTX101664
Anti-SNRPA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101664
Anti-SNRPA antibody used in IHC (Paraffin sections) (IHC-P). GTX101664
Anti-SNRPA antibody used in IHC (Paraffin sections) (IHC-P). GTX101664
Anti-SNRPA antibody used in Western Blot (WB). GTX101664
Anti-SNRPA antibody used in IHC (Paraffin sections) (IHC-P). GTX101664

Cat. No. GTX101664

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SNRPA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SNRPA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038012

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

small nuclear ribonucleoprotein polypeptide A , Mud1 , U1-A , U1A

Cellular Localization

Nucleus

Background

The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SNRPA antibody used in IHC (Paraffin sections) (IHC-P). GTX101664

GTX101664 IHC-P Image

SNRPA antibody detects SNRPA protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
SNRPA antibody (GTX101664) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SNRPA antibody used in IHC (Paraffin sections) (IHC-P). GTX101664

GTX101664 IHC-P Image

SNRPA antibody detects SNRPA protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
SNRPA antibody (GTX101664) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SNRPA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101664

GTX101664 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SNRPA(GTX101664) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-SNRPA antibody used in IHC (Paraffin sections) (IHC-P). GTX101664

GTX101664 IHC-P Image

SNRPA antibody detects SNRPA protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
SNRPA antibody (GTX101664) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SNRPA antibody used in IHC (Paraffin sections) (IHC-P). GTX101664

GTX101664 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using SNRPA(GTX101664) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SNRPA antibody used in Western Blot (WB). GTX101664

GTX101664 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNRPA antibody (GTX101664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNRPA antibody used in IHC (Paraffin sections) (IHC-P). GTX101664

GTX101664 IHC-P Image

SNRPA antibody detects SNRPA protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SNRPA antibody (GTX101664) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169