APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.37 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The immunogen used to generate this antibody corresponds to human SNX27
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888298
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sorting nexin family member 27 , MRT1 , MY014
Cellular Localization
Cytoplasm , cytosol , Early endosome
Background
This gene encodes a member of the sorting nexin family, a diverse group of cytoplasmic and membrane-associated proteins involved in endocytosis of plasma membrane receptors and protein trafficking through these compartments. All members of this protein family contain a phosphoinositide binding domain (PX domain). A highly similar protein in mouse is responsible for the specific recruitment of an isoform of serotonin 5-hydroxytryptamine 4 receptor into early endosomes, suggesting the analogous role for the human protein. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-SNX27 antibody [1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632193](/templates/default/js/lightbox/images/loading.gif "Anti-SNX27 antibody [1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632193")
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GTX632193 ICC/IF Image
SNX27 antibody [1C6] detects SNX27 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SNX27 stained by SNX27 antibody [1C6] (GTX632193) diluted at 1:200.Blue: Hoechst 33342 staining.
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GTX632193 WB Image
SNX27 antibody detects SNX27 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SNX27 antibody (GTX632193) diluted at a dilution of 1:500.
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GTX632193 ICC/IF Image
ICC/IF analysis of cos7 cells using GTX632193 SNX27 antibody [1C6].
Red : Primary antibody
Green : Polyclonal SNX27 antibody
Yellow : Merged color of red and green
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GTX632193 WB Image
WB analysis of cos7 cell transfected with SNX27 using GTX632193 SNX27 antibody [1C6].
+ : Transfected
-- : Endogenous
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REFERENCE
There are currently no references for SNX27 antibody [1C6] (GTX632193). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SNX27 antibody [1C6] (GTX632193). Be the first to share your experience with this product.
![Anti-SNX27 antibody [1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632193](/upload/website/prouct_img/small/GTX632193/GTX632193_42072_20180718_ICC_IF_w_23061202_663.webp "Anti-SNX27 antibody [1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632193")
![Anti-SNX27 antibody [1C6] used in Western Blot (WB). GTX632193](/upload/website/prouct_img/small/GTX632193/GTX632193_42072_20150709_WB_w_23061202_992.webp "Anti-SNX27 antibody [1C6] used in Western Blot (WB). GTX632193")
![Anti-SNX27 antibody [1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632193](/upload/website/prouct_img/small/GTX632193/GTX632193_20200221_ICCIF_w_23061202_930.webp "Anti-SNX27 antibody [1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632193")
![Anti-SNX27 antibody [1C6] used in Western Blot (WB). GTX632193](/upload/website/prouct_img/small/GTX632193/GTX632193_20200221_WB_w_23061202_546.webp "Anti-SNX27 antibody [1C6] used in Western Blot (WB). GTX632193")
![Anti-SNX27 antibody [1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632193](/upload/website/prouct_img/normal/GTX632193/GTX632193_42072_20180718_ICC_IF_w_23061202_663.webp "Anti-SNX27 antibody [1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632193")
![Anti-SNX27 antibody [1C6] used in Western Blot (WB). GTX632193](/upload/website/prouct_img/normal/GTX632193/GTX632193_42072_20150709_WB_w_23061202_992.webp "Anti-SNX27 antibody [1C6] used in Western Blot (WB). GTX632193")
![Anti-SNX27 antibody [1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632193](/upload/website/prouct_img/normal/GTX632193/GTX632193_20200221_ICCIF_w_23061202_930.webp "Anti-SNX27 antibody [1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632193")
![Anti-SNX27 antibody [1C6] used in Western Blot (WB). GTX632193](/upload/website/prouct_img/normal/GTX632193/GTX632193_20200221_WB_w_23061202_546.webp "Anti-SNX27 antibody [1C6] used in Western Blot (WB). GTX632193")