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SOD1 antibody [6F5]

Anti-SOD1 antibody [6F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83012
Anti-SOD1 antibody [6F5] used in Flow cytometry (FACS). GTX83012
Anti-SOD1 antibody [6F5] used in Western Blot (WB). GTX83012
Anti-SOD1 antibody [6F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83012

Cat. No. GTX83012

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6F5

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all SOD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human SOD1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11175926

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

superoxide dismutase 1 , ALS , ALS1 , HEL-S-44 , IPOA , SOD , hSod1 , homodimer

Cellular Localization

Cytoplasm,Mitochondrion,Nucleus

Background

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SOD1 antibody [6F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83012

GTX83012 ICC/IF Image

ICC/IF analysis of PANC-1 (left) and SKBR-3 (right) cells using GTX83012 SOD1 antibody [6F5].
Green : SOD1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-SOD1 antibody [6F5] used in Flow cytometry (FACS). GTX83012

GTX83012 FACS Image

FACS analysis of A431 cells using GTX83012 SOD1 antibody [6F5].
Green : SOD1
Purple : negative control

Anti-SOD1 antibody [6F5] used in Western Blot (WB). GTX83012

GTX83012 WB Image

WB analysis of HeLa (1), NIH3T3 (2), A549 (3) and A431 (4) cell lysate using GTX83012 SOD1 antibody [6F5].

Anti-SOD1 antibody [6F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83012

GTX83012 ICC/IF Image

ICC/IF analysis of 3T3-L1 cells using GTX83012 SOD1 antibody [6F5].
Green : SOD1
Blue: DRAQ5 fluorescent DNA dye

REFERENCE

Application Reference

Hathaway CK et al. Proc Natl Acad Sci U S A 2015; 112 (16) : 1183904 Endothelin-1 critically influences cardiac function via superoxide-MMP9 cascade.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399