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SOD1 antibody [AT6F1]

Anti-SOD1 antibody [AT6F1] used in Western Blot (WB). GTX57658
Anti-SOD1 antibody [AT6F1] used in Flow cytometry (FACS). GTX57658
Anti-SOD1 antibody [AT6F1] used in Western Blot (WB). GTX57658
Anti-SOD1 antibody [AT6F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57658

Cat. No. GTX57658

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT6F1

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all SOD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Product Note

Based on internal testing result, this antibody does not cross react with human SOD2.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT6F1 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SOD3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

superoxide dismutase 1 , ALS , ALS1 , HELS44 , IPOA , SOD , SOD1 , hSod1 , homodimer , superoxide dismutase 1, soluble , Cu/Zn SOD , Superoxide Dismutase 1

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SOD1 antibody [AT6F1] used in Western Blot (WB). GTX57658

GTX57658 WB Image

WB analysis of various samples using GTX57658 SOD1 antibody.
Lane 1 : Recombinant Human SOD1 protein
Lane 2 : Recombinant Human SOD2 protein
Loading : 20 ng
Dilution : 1:1000

Anti-SOD1 antibody [AT6F1] used in Flow cytometry (FACS). GTX57658

GTX57658 FACS Image

FACS analysis of Hep3B cells using GTX57658 SOD1 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

Anti-SOD1 antibody [AT6F1] used in Western Blot (WB). GTX57658

GTX57658 WB Image

WB analysis of various samples using GTX57658 SOD1 antibody.
Lane 1 : A549 whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : PC-3 whole cell lysate
Lane 5 : U87-MG whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-SOD1 antibody [AT6F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57658

GTX57658 ICC/IF Image

ICC/IF analysis of Hep3B cells using GTX57658 SOD1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

Application Reference

Osuru HP et al. Front Cardiovasc Med 2022; 9 : 711421 Molecular and Cellular Response of the Myocardium (H9C2 Cells) Towards Hypoxia and HIF-1α Inhibition.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399