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SOD1 antibody

Anti-SOD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13498
Anti-SOD1 antibody used in Western Blot (WB). GTX13498
Anti-SOD1 antibody used in Immunohistochemistry (IHC). GTX13498

Cat. No. GTX13498

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Hamster, Pig, Monkey, Xenopus laevis
Package
50 μg ($399)
See all SOD1 products

APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. IHC: Use at a concentration of 1 μg/ml. IP: Use at a concentration of 10 μg/ml. WB: Use at a concentration of 0.2 μg/ml. Detects a band of approximately 19 and 23 kDa (predicted molecular weight: 18 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

16 kDa. ( Note )

Positive Control

HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

100mM Sodium Phosphate, 50% Glycerol

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length protein (Human).

Purification

Immunogen affinity purified
This antibody was purified on an antigen coupled sepharose column.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

superoxide dismutase 1 , ALS , ALS1 , HEL-S-44 , IPOA , SOD , hSod1 , homodimer

Cellular Localization

Cytoplasm , Mitochondrion , Nucleus

Background

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SOD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13498

GTX13498 ICC/IF Image

Immunofluorescent analysis (confocal) staining of HeLa cells using GTX13498 (green); nuclei are stained in blue pseudo-color using DRAQ5.

Anti-SOD1 antibody used in Western Blot (WB). GTX13498

GTX13498 WB Image

Western blot analysis of GTX13498.
Lane 1: Rat liver tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: HeLa cell lysate.

Anti-SOD1 antibody used in Immunohistochemistry (IHC). GTX13498

GTX13498 IHC Image

Immunohistochemistry analysis of human heart tissue stained with GTX13498 at 10 ug/mL.

REFERENCE

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399