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SP1 antibody

KO/KD Orthogonal
Anti-SP1 antibody used in Western Blot (WB). GTX110593
Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110593
Anti-SP1 antibody used in ChIP assay (ChIP assay). GTX110593
Anti-SP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110593
Anti-SP1 antibody used in Immunoprecipitation (IP). GTX110593
Anti-SP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110593
Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110593
Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110593
Anti-SP1 antibody used in Western Blot (WB). GTX110593
Anti-SP1 antibody used in Western Blot (WB). GTX110593
Anti-SP1 antibody used in Western Blot (WB). GTX110593
Anti-SP1 antibody used in ChIP assay (ChIP assay). GTX110593
Anti-SP1 antibody used in Western Blot (WB). GTX110593

Cat. No. GTX110593

Reference 10
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

293T

Product Note

We do not recommend use of this product for Mouse,Rat samples.

Predict Reactivity

Mouse, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176980

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Sp1 transcription factor

Cellular Localization

Nucleus

Background

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Database

Research Area

DATA IMAGES

Anti-SP1 antibody used in Western Blot (WB). GTX110593

GTX110593 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110593

GTX110593 IHC-P Image

SP1 antibody detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SP1 stained by SP1 antibody (GTX110593) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SP1 antibody used in ChIP assay (ChIP assay). GTX110593

GTX110593 ChIP assay Image

ChIP was performed with 293T chromatin extract and 5 μg of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MGARP promoter.

Anti-SP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110593

GTX110593 ICC/IF Image

SP1 antibody detects SP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP1 stained by SP1 antibody (GTX110593) diluted at 1:100.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SP1 antibody used in Immunoprecipitation (IP). GTX110593

GTX110593 IP Image

Immunoprecipitation of SP1 protein from THP-1 whole cell extracts using 5 μg of OCT1 antibody (GTX110593).
Western blot analysis was performed using OCT1 antibody (GTX110593) diluted at 1:250.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110593

GTX110593 ICC/IF Image

SP1 antibody detects SP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP1 stained by SP1 antibody (GTX110593) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.

Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110593

GTX110593 IHC-P Image

SP1 antibody detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SP1 stained by SP1 antibody (GTX110593) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110593

GTX110593 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using SP1(GTX110593) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SP1 antibody used in Western Blot (WB). GTX110593

GTX110593 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SP1 antibody used in Western Blot (WB). GTX110593

GTX110593 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SP1 antibody used in Western Blot (WB). GTX110593

GTX110593 WB Image

KO/KD

The data was published in the journal Cells in 2020. PMID: 32290085

Anti-SP1 antibody used in ChIP assay (ChIP assay). GTX110593

GTX110593 ChIP assay Image

The data was published in the journal Pharmacol Res Perspect in 2018. PMID: 30455960

Anti-SP1 antibody used in Western Blot (WB). GTX110593

GTX110593 WB Image

The data was published in the 2017 in Oxid Med Cell Longev. PMID: 28740570

REFERENCE

Application Reference

H Nishida et al. Cancer Res Commun 2024; HDAC inhibition induces CD26 expression on myeloma cells via the c-Myc/Sp1-mediated promoter activation
Application : ChIP assay
Wu R et al. J Biol Chem 2023; 299 (9) : 105153 Cooperation of long noncoding RNA LOC100909675 and transcriptional regulator CTCF modulates Cdk1 transcript to control astrocyte proliferation.
Moon SJ et al. Cancers (Basel) 2023; 15 (4) CTTN Overexpression Confers Cancer Stem Cell-like Properties and Trastuzumab Resistance via DKK-1/WNT Signaling in HER2 Positive Breast Cancer.
Application : WB
Reactivity : Human
Gonz?lez-Gonz?lez L et al. Tissue Barriers 2022; 10 (2) : 1994351 ZO-2 favors Hippo signaling, and its re-expression in the steatotic liver by AMPK restores junctional sealing.
Li Y et al. J Nanobiotechnology 2022; 20 (1) : 39 Betulinic acid self-assembled nanoparticles for effective treatment of glioblastoma.
Application : WB
Show More +
Submit a Reference

REVIEW

SP1 antibody Cat. No. GTX110593

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Anti-SP1 antibody used in Western Blot (WB). GTX110593
Date : Anonymous submitted on 09-Aug-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : OVSAYO cells
Blocking : 3% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Anti-SP1 antibody used in Western Blot (WB). GTX110593
Date : Anonymous submitted on 30-Mar-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC3 cells
Amount used : 20μg
Blocking : 5% milk, 4°C, 1Hr
Primary Antibody : 1:3000, 4°C, 12Hr
Anti-SP1 antibody used in Western Blot (WB). GTX110593
Date : Anonymous submitted on 09-Jan-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Raji cells
Amount used : 50μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Submit a Review
Application Reference
H Nishida et al. Cancer Res Commun 2024; HDAC inhibition induces CD26 expression on myeloma cells via the c-Myc/Sp1-mediated promoter activation
Application : ChIP assay
Wu R et al. J Biol Chem 2023; 299 (9):105153 Cooperation of long noncoding RNA LOC100909675 and transcriptional regulator CTCF modulates Cdk1 transcript to control astrocyte proliferation.
Moon SJ et al. Cancers (Basel) 2023; 15 (4) CTTN Overexpression Confers Cancer Stem Cell-like Properties and Trastuzumab Resistance via DKK-1/WNT Signaling in HER2 Positive Breast Cancer.
Application : WB
Reactivity : Human
Gonz?lez-Gonz?lez L et al. Tissue Barriers 2022; 10 (2):1994351 ZO-2 favors Hippo signaling, and its re-expression in the steatotic liver by AMPK restores junctional sealing.
Li Y et al. J Nanobiotechnology 2022; 20 (1):39 Betulinic acid self-assembled nanoparticles for effective treatment of glioblastoma.
Application : WB
Lixia Liu et al. Mol Cancer Ther 2021; 20 (10):1880-1892 Hsp90 Inhibitor STA9090 Sensitizes Hepatocellular Carcinoma to Hyperthermia-Induced DNA Damage by Suppressing DNA-PKcs Protein Stability and mRNA Transcription.
Application : ICC/IF
Reactivity : Human
Chien SY et al. Cells 2020; 9 (4) Noggin Inhibits IL-1β and BMP-2 Expression, and Attenuates Cartilage Degeneration and Subchondral Bone Destruction in Experimental Osteoarthritis.
Lee J et al. Invest Ophthalmol Vis Sci 2020; 61 (8):41 ZEB1 Mediates Fibrosis in Corneal Endothelial Mesenchymal Transition Through SP1 and SP3.
Application : WB
Reactivity : Human
Seira N et al. Pharmacol Res Perspect 2018; 6 (6):e00441 Cellular density-dependent increases in HIF-1α compete with c-Myc to down-regulate human EP4 receptor promoter activity through Sp-1-binding region.
Application : IP
Reactivity : Human
Carrasco-Torres G et al. Oxid Med Cell Longev 2017; 2017:4674918 Quercetin reverses rat liver preneoplastic lesions induced by chemical carcinogenesis.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169