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SP3 antibody

KO/KD Orthogonal Overexpression
Anti-SP3 antibody used in Western Blot (WB). GTX129426
Anti-SP3 antibody used in Immunoprecipitation (IP). GTX129426
Anti-SP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX129426
Anti-SP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX129426
Anti-SP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX129426
Anti-SP3 antibody used in Western Blot (WB). GTX129426
Anti-SP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129426
Anti-SP3 antibody used in Western Blot (WB). GTX129426
Anti-SP3 antibody used in Western Blot (WB). GTX129426

Cat. No. GTX129426

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

Juarkat , DDDDK-tagged SP3-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885989

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Sp3 transcription factor , SPR2

Cellular Localization

Nucleus

Background

This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SP3 antibody used in Western Blot (WB). GTX129426

GTX129426 WB Image

KO/KD

Wild-type (WT) and SP3 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP3 antibody (GTX129426) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SP3 antibody used in Immunoprecipitation (IP). GTX129426

GTX129426 IP Image

Immunoprecipitation of SP3 protein from Jurkat nuclear extracts using 5 μg of SP3 antibody (GTX129426).
Western blot analysis was performed using SP3 antibody (GTX129426) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX129426

GTX129426 IHC-P Image

SP3 antibody detects SP3 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
SP3 antibody (GTX129426) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX129426

GTX129426 IHC-P Image

SP3 antibody detects SP3 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SP3 antibody (GTX129426) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX129426

GTX129426 IHC-P Image

SP3 antibody detects SP3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SP3 stained by SP3 antibody (GTX129426) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SP3 antibody used in Western Blot (WB). GTX129426

GTX129426 WB Image

Orthogonal

SP3 antibody detects SP3 protein by western blot analysis.
A. 30 μg Jurkat whole cell extract
B. 30 μg Jurkat nuclear extract
7.5 % SDS-PAGE
SP3 antibody (GTX129426) dilution: 1:1000

Anti-SP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129426

GTX129426 ICC/IF Image

SP3 antibody detects SP3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP3 stained by SP3 antibody (GTX129426) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-SP3 antibody used in Western Blot (WB). GTX129426

GTX129426 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP3 antibody (GTX129426) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SP3 antibody used in Western Blot (WB). GTX129426

GTX129426 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP3 antibody (GTX129426) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lee J et al. Invest Ophthalmol Vis Sci 2020; 61 (8) : 41 ZEB1 Mediates Fibrosis in Corneal Endothelial Mesenchymal Transition Through SP1 and SP3.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169