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SPAM1 antibody

Anti-SPAM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54679
Anti-SPAM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54679
Anti-SPAM1 antibody used in Western Blot (WB). GTX54679

Cat. No. GTX54679

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all SPAM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sperm adhesion molecule 1 , HEL-S-96n , HYA1 , HYAL1 , HYAL3 , HYAL5 , PH-20 , PH20 , SPAG15

Background

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-SPAM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54679

GTX54679 IHC-P Image

IHC-P analysis of rat testis tissue using GTX54679 SPAM1 antibody.
Dilution : 1:100

Anti-SPAM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54679

GTX54679 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54679 SPAM1 antibody.
Blue : DAPI

Anti-SPAM1 antibody used in Western Blot (WB). GTX54679

GTX54679 WB Image

WB analysis of various sample lysates using GTX54679 SPAM1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399