GeneTex

  • Country / Location Selection

United States (US)

SPARTIN antibody, Internal

KO/KD
Anti-SPARTIN antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX88225
Anti-SPARTIN antibody, Internal used in Western Blot (WB). GTX88225

Cat. No. GTX88225

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μg ($399)
See all SPARTIN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88225-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EASGTDVKQLDQGNK, from the internal region of the protein sequence according to NP_055902.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

spartin , SPG20 , TAHCCP1

Cellular Localization

Cytoplasm

Background

This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome). [provided by RefSeq, Nov 2008]

Database

Research Area

DATA IMAGES

Anti-SPARTIN antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX88225

GTX88225 ICC/IF Image

KO/KD

ICC/IF analysis of methanol-fixed HeLa before (left) and after (right) introduction of siRNA using GTX88225 SPARTIN antibody, Internal.
Dilution : 2μg/ml

Anti-SPARTIN antibody, Internal used in Western Blot (WB). GTX88225

GTX88225 WB Image

WB analysis of HeLa lysate using GTX88225 SPARTIN antibody, Internal.
Dilution : 2μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

There are currently no references for SPARTIN antibody, Internal (GTX88225). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for SPARTIN antibody, Internal (GTX88225). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399