GeneTex

  • Country / Location Selection

United States (US)

SPIB antibody [4G5]

Anti-SPIB antibody [4G5] used in IHC (Paraffin sections) (IHC-P). GTX60635
Anti-SPIB antibody [4G5] used in IHC (Paraffin sections) (IHC-P). GTX60635
Anti-SPIB antibody [4G5] used in Flow cytometry (FACS). GTX60635
Anti-SPIB antibody [4G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60635
Anti-SPIB antibody [4G5] used in ELISA (ELISA). GTX60635
Anti-SPIB antibody [4G5] used in Western Blot (WB). GTX60635

Cat. No. GTX60635

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4G5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($409)
See all SPIB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human SPIB (AA: 200-252) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887992

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Spi-B transcription factor , SPI-B

Background

The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-SPIB antibody [4G5] used in IHC (Paraffin sections) (IHC-P). GTX60635

GTX60635 IHC-P Image

IHC-P analysis of ovarian cancer tissue using GTX60635 SPIB antibody [4G5].

Anti-SPIB antibody [4G5] used in IHC (Paraffin sections) (IHC-P). GTX60635

GTX60635 IHC-P Image

IHC-P analysis of esophageal cancer using GTX60635 SPIB antibody [4G5].

Anti-SPIB antibody [4G5] used in Flow cytometry (FACS). GTX60635

GTX60635 FACS Image

FACS analysis of NIH3T3 cells using GTX60635 SPIB antibody [4G5].
Green : SPIB
Red : negative control

Anti-SPIB antibody [4G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60635

GTX60635 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60635 SPIB antibody [4G5].
Green : SPIB
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-SPIB antibody [4G5] used in ELISA (ELISA). GTX60635

GTX60635 ELISA Image

ELISA analysis of antigen using GTX60635 SPIB antibody [4G5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-SPIB antibody [4G5] used in Western Blot (WB). GTX60635

GTX60635 WB Image

WB analysis of A549 (1), PC-3 (2), and NIH3T3 (3) cell lysate using GTX60635 SPIB antibody [4G5].

REFERENCE

Application Reference

Ho YJ et al. Acta Histochem 2015; (Epub) Tissue microarray-based study of hepatocellular carcinoma validating SPIB as potential clinical prognostic marker.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for SPIB antibody [4G5] (GTX60635). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 409