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SRC1 antibody [1135/H4]

Anti-SRC1 antibody [1135/H4] used in Western Blot (WB). GTX15795
Anti-SRC1 antibody [1135/H4] used in Western Blot (WB). GTX15795

Cat. No. GTX15795

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1135/H4

Isotype

 IgG1

Application

 WB, IHC-P, FACS, IP, ChIP assay, IHC

Reactivity

 Human, Mouse, Rat, Primate
Package
100 μl ($399)
See all SRC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
IHC-P 1:200
FACS 1μg per 10⁶ cells
IP Assay dependent
ChIP assay 5μl/10⁶ cells
IHC Assay dependent
Not tested in other applications.

Calculated MW

157 kDa. ( Note )

Product Note

The epitope for GTX15795 has been mapped to the extreme C-terminal end of this fragment.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues 477-947 of human SRC-1.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor coactivator 1 , F-SRC-1 , KAT13A , RIP160 , SRC1 , bHLHe42 , bHLHe74

Cellular Localization

Nucleus

Background

The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SRC1 antibody [1135/H4] used in Western Blot (WB). GTX15795

GTX15795 WB Image

WB analysis of COS cells using GTX15795 SRC1 antibody [1135/H4].

Anti-SRC1 antibody [1135/H4] used in Western Blot (WB). GTX15795

GTX15795 WB Image

WB analysis of 25 ug of LNCaP (lane 1) and PC-3 (lane 2) using GTX15795 SRC1 antibody [1135/H4].
Dilution : 1:2000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399