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STAT1 antibody [SD20-75]

Recombinant antibody
Anti-STAT1 antibody [SD20-75] used in Western Blot (WB). GTX01142
Anti-STAT1 antibody [SD20-75] used in Flow cytometry (FACS). GTX01142

Cat. No. GTX01142

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 SD20-75

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human
Package
100 μl ($399)
See all STAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal transducer and activator of transcription 1 , CANDF7 , IMD31A , IMD31B , IMD31C , ISGF3 , STAT1 , STAT91

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-STAT1 antibody [SD20-75] used in Western Blot (WB). GTX01142

GTX01142 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT1 antibody [SD20-75] (GTX01142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT1 antibody [SD20-75] used in Flow cytometry (FACS). GTX01142

GTX01142 FACS Image

FACS analysis of MCF-7 cells using GTX01142 STAT1 antibody [SD20-75].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399