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STAT2 antibody [C2C3], C-term

Orthogonal IP/MS
Anti-STAT2 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX103117
Anti-STAT2 antibody [C2C3], C-term used in Western Blot (WB). GTX103117
Anti-STAT2 antibody [C2C3], C-term used in Western Blot (WB). GTX103117
Anti-STAT2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX103117
Anti-STAT2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX103117
Anti-STAT2 antibody [C2C3], C-term used in Western Blot (WB). GTX103117

Cat. No. GTX103117

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all STAT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , THP-1

Product Note

IP/MS validation was supported by references (PMID:30377386)

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human STAT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164877

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal transducer and activator of transcription 2 , IMD44 , ISGF-3 , P113 , STAT113

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-STAT2 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX103117

GTX103117 IP Image

STAT2 antibody immunoprecipitates STAT2 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of STAT2 protein by 2 μg of STAT2 antibody (GTX103117) 7.5% SDS-PAGE The immunoprecipitated STAT2 protein was detected by STAT2 antibody (GTX103117) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-STAT2 antibody [C2C3], C-term used in Western Blot (WB). GTX103117

GTX103117 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with STAT2 antibody [C2C3], C-term (GTX103117) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT2 antibody [C2C3], C-term used in Western Blot (WB). GTX103117

GTX103117 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with STAT2 antibody [C2C3], C-term (GTX103117) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-STAT2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX103117

GTX103117 IHC-P Image

STAT2 antibody [C2C3], C-term detects STAT2 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
STAT2 antibody [C2C3], C-term (GTX103117) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-STAT2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX103117

GTX103117 IHC-P Image

STAT2 antibody [C2C3], C-term detects STAT2 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
STAT2 antibody [C2C3], C-term (GTX103117) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-STAT2 antibody [C2C3], C-term used in Western Blot (WB). GTX103117

GTX103117 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
D: THP-1
5% SDS PAGE
GTX103117 diluted at 1:2000

REFERENCE

Application Reference

Iris E Glykofridis et al. Elife 2021; 10 Loss of FLCN-FNIP1/2 induces a non-canonical interferon response in human renal tubular epithelial cells.
Application : WB
Reactivity : Human
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Koh EH et al. Diabetes 2018; (Epub) mir-30a remodels subcutaneous adipose tissue inflammation to improve * sensitivity in obesity.
Application : WB
Reactivity : Mouse
Yu JS et al. Sci Rep 2017; 7 : 45171 schisandrin a inhibits dengue viral replication via upregulating antiviral * responses through stat signaling pathway.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169