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STAT3 antibody [9D8]

KO/KD Orthogonal
Anti-STAT3 antibody [9D8] used in Western Blot (WB). GTX15789
Anti-STAT3 antibody [9D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15789
Anti-STAT3 antibody [9D8] used in Immunoprecipitation (IP). GTX15789
Anti-STAT3 antibody [9D8] used in IHC (Paraffin sections) (IHC-P). GTX15789
Anti-STAT3 antibody [9D8] used in ChIP assay (ChIP assay). GTX15789

Cat. No. GTX15789

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9D8

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Primate
Package
100 μl ($399)
See all STAT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000
ICC/IF 1:100
IHC-P 1:1600
IP 2 μg
ChIP assay 1-3 μl
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Residues 655-770 from human recombinant protein expressed in bacteria

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_2887522

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal transducer and activator of transcription 3 , ADMIO , ADMIO1 , APRF , HIES

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-STAT3 antibody [9D8] used in Western Blot (WB). GTX15789

GTX15789 WB Image

KO/KD

WB analysis of STAT3 siRNA transfected or non-targeting control transfected U2OS cell lysate using GTX15789 STAT3 antibody [9D8].
Dilution : 1:1000
Loading : 25 μg

Anti-STAT3 antibody [9D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15789

GTX15789 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15789 STAT3 antibody [9D8].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

Anti-STAT3 antibody [9D8] used in Immunoprecipitation (IP). GTX15789

GTX15789 IP Image

IP analysis of HepG2 cell lysates using GTX15789 STAT3 antibody [9D8].
IP reaction : 2μg antibody / 750μg lysate
Dilution : 1:5000

Anti-STAT3 antibody [9D8] used in IHC (Paraffin sections) (IHC-P). GTX15789

GTX15789 IHC-P Image

IHC-P analysis of biopsies of normal and cancer tissues using GTX15789 STAT3 antibody [9D8].
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95ºC
Dilution : 1:1600

Anti-STAT3 antibody [9D8] used in ChIP assay (ChIP assay). GTX15789

GTX15789 ChIP assay Image

Orthogonal

ChIP analysis of 1x10⁶ HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes using GTX15789 STAT3 antibody [9D8]. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to exon-1 or exon-28 of the LAMC1 gene.
IP reaction : 1.0ul antibody / 100ul sample

REFERENCE

Application Reference

Lee YK et al. Cancers (Basel) 2020; 12 (9) Mitochondrial Respiratory Defect Enhances Hepatoma Cell Invasiveness via STAT3/NFE2L1/STX12 Axis.
Gao J et al. Cancer Biomark 2018; (Epub) Targetedregulationof STAT3 by miR-29a in mediating * resistance of nasopharyngeal carcinoma cell line CNE-1.
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399