STAT3 antibody [HL1134]

Name: STAT3 antibody [HL1134]
Brand: GeneTex
SKU: GTX636400
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-STAT3 antibody [HL1134] used in Western Blot (WB). GTX636400

Anti-STAT3 antibody [HL1134] used in IHC (Paraffin sections) (IHC-P). GTX636400

Cat. No. GTX636400

Reference 2

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1134
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat, Cat, Dog
Package	100 μl ($399), 25 μl ($169)

See all STAT3 products

Datasheet
Reference

View product citations for antibody GTX636400 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Japanese Medaka(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human STAT3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909976

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal transducer and activator of transcription 3 , ADMIO , ADMIO1 , APRF , HIES

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

Database

Gene ID: 6774 STAT3
UniProt: P40763 STAT3

Research Area

DATA IMAGES

GTX636400 WB Image

Wild-type (WT) and STAT3 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [HL1134] (GTX636400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636400 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with STAT3 antibody [HL1134] (GTX636400) diluted at 1:1000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX636400 IHC-P Image

STAT3 antibody [HL1134] detects STAT3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
STAT3 stained by STAT3 antibody [HL1134] (GTX636400) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636400 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [HL1134] (GTX636400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636400 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [HL1134] (GTX636400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Shen Y et al. Molecules 2023; 29 (1) Transformation of Ginsenosides by Lactiplantibacillus plantarum MB11 Fermentation: Minor Ginsenosides Conversion and Enhancement of Anti-Colorectal Cancer Activity.

Yunjiao Shen et al. Authorea. 2023; Transformation of ginsenosides by Lactiplantibacillus plantarum MB11 fermentation: minor ginsenosides conversion and enhancement of anti-colorectal cancer activity

Application : WB

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