APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 1 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
A20 Cell Lysate(GTX27180) , Human Spleen Tissue Lysate(GTX27921) , Human Spleen Tissue Slide
Product Note
This antibody is specific for STIM2 C-Terminus
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
STIM2 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human STIM2.The immunogen is located within the last 50 amino acids of STIM2.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
stromal interaction molecule 2
Cellular Localization
Endoplasmic reticulum membrane
Background
In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least three isoforms of STIM2 are known to exist. This STIM2 antibody is predicted to have no cross-reactivity to STIM1.
Database
DATA IMAGES
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GTX85441 IHC-P Image
IHC-P analysis of human spleen tissue using GTX85441 STIM2 antibody.
Working concentration : 2.5 μg/ml
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GTX85441 WB Image
WB analysis of A-20 cell lysate using GTX85441 STIM2 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml
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GTX85441 IHC-P Image
IHC-P analysis of human spleen tissue using GTX85441 STIM2 antibody.
Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
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