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STK39 antibody [2E10]

Overexpression
Anti-STK39 antibody [2E10] used in IHC (Paraffin sections) (IHC-P). GTX83543
Anti-STK39 antibody [2E10] used in Western Blot (WB). GTX83543
Anti-STK39 antibody [2E10] used in IHC (Paraffin sections) (IHC-P). GTX83543
Anti-STK39 antibody [2E10] used in IHC (Paraffin sections) (IHC-P). GTX83543
Anti-STK39 antibody [2E10] used in IHC (Paraffin sections) (IHC-P). GTX83543
Anti-STK39 antibody [2E10] used in IHC (Paraffin sections) (IHC-P). GTX83543
Anti-STK39 antibody [2E10] used in IHC (Paraffin sections) (IHC-P). GTX83543
Anti-STK39 antibody [2E10] used in IHC (Paraffin sections) (IHC-P). GTX83543
Anti-STK39 antibody [2E10] used in Western Blot (WB). GTX83543

Cat. No. GTX83543

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2E10

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Drosophila
Package
100 μl ($519)
See all STK39 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human STK39 expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10726978

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine/threonine kinase 39 , DCHT , PASK , SPAK

Cellular Localization

Cytoplasm,Nucleus

Background

May act as a mediator of stress-activated signals.

Database

Research Area

DATA IMAGES

Anti-STK39 antibody [2E10] used in IHC (Paraffin sections) (IHC-P). GTX83543

GTX83543 IHC-P Image

IHC-P analysis of pancreas tissue using GTX83543 STK39 antibody [2E10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-STK39 antibody [2E10] used in Western Blot (WB). GTX83543

GTX83543 WB Image

WB analysis of various cell lines using GTX83543 STK39 antibody [2E10].
Loading : 35 ug per lane

Anti-STK39 antibody [2E10] used in IHC (Paraffin sections) (IHC-P). GTX83543

GTX83543 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX83543 STK39 antibody [2E10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-STK39 antibody [2E10] used in IHC (Paraffin sections) (IHC-P). GTX83543

GTX83543 IHC-P Image

IHC-P analysis of colon tissue using GTX83543 STK39 antibody [2E10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-STK39 antibody [2E10] used in IHC (Paraffin sections) (IHC-P). GTX83543

GTX83543 IHC-P Image

IHC-P analysis of endometrium tissue using GTX83543 STK39 antibody [2E10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-STK39 antibody [2E10] used in IHC (Paraffin sections) (IHC-P). GTX83543

GTX83543 IHC-P Image

IHC-P analysis of ovary tissue using GTX83543 STK39 antibody [2E10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-STK39 antibody [2E10] used in IHC (Paraffin sections) (IHC-P). GTX83543

GTX83543 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83543 STK39 antibody [2E10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-STK39 antibody [2E10] used in IHC (Paraffin sections) (IHC-P). GTX83543

GTX83543 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX83543 STK39 antibody [2E10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-STK39 antibody [2E10] used in Western Blot (WB). GTX83543

GTX83543 WB Image

Overexpression

WB analysis of HEK293T cells transfected with STK39 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83543 STK39 antibody [2E10].
Loading : 5 ug per lane

REFERENCE

Application Reference

Yarikipati P et al. PLoS Genet 2023; 19 (10) : e1010975 Unanticipated domain requirements for Drosophila Wnk kinase in vivo.
JM Humphreys et al. Mol Biol Cell 2023; Hydrostatic Pressure Sensing by WNK kinases
Application : WB
Reactivity : Drosophila
John M Pleinis et al. Am J Physiol Cell Physiol 2021; 320 (5) : C703-C721 WNKs are potassium-sensitive kinases.
Application : WB
Reactivity : Drosophila
Sun Q et al. J Am Soc Nephrol 2018; 29 (5) : 1449-1461 Intracellular Chloride and Scaffold Protein Mo25 Cooperatively Regulate Transepithelial Ion Transport through WNK Signaling in the Malpighian Tubule.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519