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SUCLA2 antibody [N2C3]

KO/KD
Anti-SUCLA2 antibody [N2C3] used in Western Blot (WB). GTX109728
Anti-SUCLA2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX109728
Anti-SUCLA2 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109728
Anti-SUCLA2 antibody [N2C3] used in Western Blot (WB). GTX109728
Anti-SUCLA2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX109728
Anti-SUCLA2 antibody [N2C3] used in Western Blot (WB). GTX109728

Cat. No. GTX109728

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SUCLA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Mouse brain , HepG2 , Molt-4 , HeLa

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SUCLA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952096

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

succinate-CoA ligase ADP-forming beta subunit , A-BETA , A-SCS , MTDPS5 , SCS-betaA

Cellular Localization

Mitochondrion

Background

Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SUCLA2 antibody [N2C3] used in Western Blot (WB). GTX109728

GTX109728 WB Image

KO/KD

Wild-type (WT) and SUCLA2 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLA2 antibody [N2C3] (GTX109728) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SUCLA2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX109728

GTX109728 IHC-P Image

SUCLA2 antibody [N2C3] detects SUCLA2 protein at cytoplasm on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
SUCLA2 antibody [N2C3] (GTX109728) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SUCLA2 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109728

GTX109728 ICC/IF Image

SUCLA2 antibody [N2C3] detects SUCLA2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: SUCLA2 protein stained by SUCLA2 antibody [N2C3] (GTX109728) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-SUCLA2 antibody [N2C3] used in Western Blot (WB). GTX109728

GTX109728 WB Image

Sample (20 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX109728 diluted at 1:20000

Anti-SUCLA2 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX109728

GTX109728 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SUCLA2(GTX109728) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SUCLA2 antibody [N2C3] used in Western Blot (WB). GTX109728

GTX109728 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: Molt-4 (GTX27912)
10% SDS PAGE
GTX109728 diluted at 1:1000

REFERENCE

Application Reference

Zhao Y et al. Sci Rep 2017; 7 (1) : 7169 Loss of succinyl-CoA synthase ADP-forming β subunit disrupts mtDNA stability and mitochondrial dynamics in neurons.
Application : WB
Reactivity : Mouse
Piel RB 3rd et al. Biochemistry 2016; 55 (37) : 5204-17 A Novel Role for Progesterone Receptor Membrane Component 1 (PGRMC1): A Partner and Regulator of Ferrochelatase.
Application : WB
Reactivity : Human
Medlock AE et al. PLoS One 2015; 10 (8) : e0135896 Identification of the Mitochondrial Heme Metabolism Complex.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169