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SUPV3L1 antibody [GT244]

KO/KD Overexpression
Anti-SUPV3L1 antibody [GT244] used in Western Blot (WB). GTX628260
Anti-SUPV3L1 antibody [GT244] used in IHC (Paraffin sections) (IHC-P). GTX628260
Anti-SUPV3L1 antibody [GT244] used in Western Blot (WB). GTX628260
Anti-SUPV3L1 antibody [GT244] used in Western Blot (WB). GTX628260
Anti-SUPV3L1 antibody [GT244] used in Western Blot (WB). GTX628260

Cat. No. GTX628260

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT244

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SUPV3L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

H1299 , HCT116 , HeLa , GFP-human SUV3-transfected HeLa cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human SUPV3L1

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888028

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Suv3 like RNA helicase , SUV3

Cellular Localization

Mitochondrion matrix , Nucleus

Background

ATPase and DNA/RNA helicase able to unwind DNA/DNA, DNA/RNA and RNA/RNA duplexes in the 5'-3' direction. May protect cells from apoptosis.

Database

Research Area

DATA IMAGES

Anti-SUPV3L1 antibody [GT244] used in Western Blot (WB). GTX628260

GTX628260 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUPV3L1 antibody [GT244] (GTX628260) diluted at 1:500.

Anti-SUPV3L1 antibody [GT244] used in IHC (Paraffin sections) (IHC-P). GTX628260

GTX628260 IHC-P Image

SUPV3L1 antibody [GT244] detects SUV3 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
SUPV3L1 antibody [GT244] (GTX628260) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SUPV3L1 antibody [GT244] used in Western Blot (WB). GTX628260

GTX628260 WB Image

SUPV3L1 antibody [GT0003] detects SUV3 protein by Western blot analysis.
A. 30 μg H1299 whole cell lysate/extract
B. 30 μg HCT116 whole cell lysate/extract
7.5 % SDS-PAGE
SUPV3L1 antibody [GT0003] (GTX628260) dilution: 1:1000

Anti-SUPV3L1 antibody [GT244] used in Western Blot (WB). GTX628260

GTX628260 WB Image

Overexpression

SUPV3L1 antibody [GT0003] detects SUV3 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg whole cell lysate/extract of GFP-human SUV3-transfected HeLa cells
7.5 % SDS-PAGE
SUPV3L1 antibody [GT0003] (GTX628260) dilution: 1:5000

Anti-SUPV3L1 antibody [GT244] used in Western Blot (WB). GTX628260

GTX628260 WB Image

Overexpression

The data was published in the journal Nat Commun in 2018.PMID: 29967381

REFERENCE

Application Reference

Pietras Z et al. Nat Commun 2018; 9 (1) : 2558 Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations