GeneTex

  • Country / Location Selection

United States (US)

SV40 T-antigen antibody

Anti-SV40 T-antigen antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134378
Anti-SV40 T-antigen antibody used in Western Blot (WB). GTX134378

Cat. No. GTX134378

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Simian virus 40
Package
100 μl ($399),
25 μl ($169)
See all SV40 T-antigen products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

293T

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Simian virus 40

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of SV40 T-antigen (SV40 virus (strain 776)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887264

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Simian virus 40 T antigen ( Large T) , SV40 virus T antigen ( Large T)

Research Area

DATA IMAGES

Anti-SV40 T-antigen antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134378

GTX134378 ICC/IF Image

SV40 T-antigen antibody detects SV40 T-antigen protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SV40 T-antigen stained by SV40 T-antigen antibody (GTX134378) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Anti-SV40 T-antigen antibody used in Western Blot (WB). GTX134378

GTX134378 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SV40 Large T antigen antibody (GTX134378) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Rosemarie Q et al. mBio 2023; 14 (1) : e0268622 How Epstein-Barr Virus Induces the Reorganization of Cellular Chromatin.
Application : ICC/IF
Submit a Reference

REVIEW

There are currently no reviews for SV40 T-antigen antibody (GTX134378). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169