APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , NCI-H929 , 293T , A431 , HeLa , HepG2
Predict Reactivity
Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human SWAP70. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885389
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
switching B cell complex subunit SWAP70 , HSPC321 , SWAP-70
Cellular Localization
Cytoplasm , Cell membrane , Nucleus , Cell projection , lamellipodium
Background
Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
Database
Research Area
DATA IMAGES
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GTX115667 IHC-P Image
SWAP70 antibody detects SWAP70 protein at cytosol on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
SWAP70 antibody (GTX115667) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115667 ICC/IF Image
SWAP70 antibody detects SWAP70 protein at cytoplasm by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SWAP70 protein stained by SWAP70 antibody (GTX115667) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX115667 WB Image
SWAP70 antibody detects SWAP70 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SWAP70 antibody (GTX115667) diluted at a dilution of 1:10000.
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GTX115667 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SWAP70 antibody (GTX115667) diluted at 1:10000 and competitor's antibody (Cat. No. 654702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX115667 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SWAP70 antibody (GTX115667) diluted at 1:10000 and competitor's antibody (Cat. No. 654702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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REFERENCE
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REVIEW
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