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SYNPO2 antibody

Anti-SYNPO2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85139
Anti-SYNPO2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85139
Anti-SYNPO2 antibody used in Western Blot (WB). GTX85139

Cat. No. GTX85139

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all SYNPO2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

Positive Control

Human Skeletal Muscle Tissue Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SYNPO2 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human SYNPO2.The immunogen is located within amino acids 320 - 370 of SYNPO2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptopodin 2

Cellular Localization

Nucleus,Cytoplasm,Cytoplasm, cytoskeleton

Background

SYNPO2 was initially identified as myopodin, a member of the synaptopodin family that contains one PPXY motif and multiple PXXP motifs. It colocalizes with alpha-actinin and is found at the Z-disc and during stress conditions will translocate to the nucleus, suggesting that it is part of signaling pathways in addition to its function as a structural protein. SYNPO2 has been shown to bind to calmodulin, alpha-actinin, and smooth muscle myosin and will stimulate actin polymerization in a calmodulin dependent manner, consistent with its proposed role in organizing the cytoskeleton. While deletion of SYNPO2 has been reported to be highly correlated with the invasiveness of prostate cancers, other reports suggest that down-regulation of SYNPO2 reduces the invasiveness and motility of prostate cancer cells.

Database

Research Area

DATA IMAGES

Anti-SYNPO2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85139

GTX85139 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX85139 SYNPO2 antibody.
Working concentration : 20 μg/ml

Anti-SYNPO2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85139

GTX85139 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX85139 SYNPO2 antibody.
Working concentration : 5 μg/ml

Anti-SYNPO2 antibody used in Western Blot (WB). GTX85139

GTX85139 WB Image

WB analysis of human skeletal muscle tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX85139 SYNPO2 antibody.
Working concentration : 1 μg/ml

REFERENCE

Application Reference

Salim C et al. J Orofac Orthop 2021; Role of chaperone-assisted selective autophagy (CASA) in mechanical stress protection of periodontal ligament cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399