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Salmonella enteritidis LPS antibody [se-1]

Anti-Salmonella enteritidis LPS antibody [se-1] used in Dot blot (Dot). GTX00861
Anti-Salmonella enteritidis LPS antibody [se-1] used in ELISA (ELISA). GTX00861
Anti-Salmonella enteritidis LPS antibody [se-1] used in (). GTX00861
Anti-Salmonella enteritidis LPS antibody [se-1] used in ELISA (ELISA). GTX00861
Anti-Salmonella enteritidis LPS antibody [se-1] used in Western Blot (WB). GTX00861
Anti-Salmonella enteritidis LPS antibody [se-1] used in Western Blot (WB). GTX00861
Anti-Salmonella enteritidis LPS antibody [se-1] used in Lateral Flow (Lateral Flow). GTX00861

Cat. No. GTX00861

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 se-1

Isotype

 IgG1

Application

 WB, Dot, ELISA, Lateral Flow

Reactivity

 Salmonella typhimurium, Salmonella Enteritidis
Package
100 μg ($399)
See all Salmonella LPS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
Dot Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Not tested in other applications.

Product Note

It does not react with other gram-negative food-poisoning bacteria like E. coli, Vibrio parahaemolyticus, and Campylobacter species.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Salmonella Enteritidis

Immunogen

Crude extract of Salmonella Enteritidis

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Salmonella enteritidis LPS antibody [se-1] used in Dot blot (Dot). GTX00861

GTX00861 Dot Image

Extracts of each strain of food poisoning bacteria were coated onto 5 area of a nitrocellulose membrane and reacted with GTX00861 Salmonella enteritidis LPS antibody [se-1].
GTX00861 recognizes LPS of S. Enteritidis, but does not react with any non-Salmonella bacteria tested.
SE: S. Enteritidis
EC: E. coli
CJ: Campylobacter jejuni
VP: V. parahaemolyticus.

Anti-Salmonella enteritidis LPS antibody [se-1] used in ELISA (ELISA). GTX00861

GTX00861 ELISA Image

ELISA analysis of crude extract of S. Enteritidis using GTX00861 Salmonella enteritidis LPS antibody [se-1].
Protein coating amount : 100μl per well at indicated amount
Antibody amount : 100μl (1:500) per well

Anti-Salmonella enteritidis LPS antibody [se-1] used in (). GTX00861

GTX00861 Image

Reactivity of GTX00861 Salmonella enteritidis LPS antibody [se-1] with various food poisoning bacteria.

Anti-Salmonella enteritidis LPS antibody [se-1] used in ELISA (ELISA). GTX00861

GTX00861 ELISA Image

ELISA analysis of crude extract of S. Enteritidis using GTX00861 Salmonella enteritidis LPS antibody [se-1].
Protein coating amount : 100μl (1 μg/ml) per well
Antibody amount : 100μl at indicated dilution

Anti-Salmonella enteritidis LPS antibody [se-1] used in Western Blot (WB). GTX00861

GTX00861 WB Image

WB analysis of several food poisoning bacteria using GTX00861 Salmonella enteritidis LPS antibody [se-1]. This antibody reacts only with S. Enteritidis.
Lane 1 : S. Enteritidis
Lane 2 : Vibrio parahaemolyticus
Lane 3 : Escherichia coli
Lane 4 : E. coli O157:H7

Anti-Salmonella enteritidis LPS antibody [se-1] used in Western Blot (WB). GTX00861

GTX00861 WB Image

WB analysis of various samples using GTX00861 Salmonella enteritidis LPS antibody [se-1]. They showed similar band patterns characteristic of LPS, O-chain repeating unit. MAb (se-01) recognizes common antigenic determinants that are found in the structural components of Salmonella LPS.
Lane 1 : Crude extract of S. Enteritidis cells
Lane 2 : Purified LPS of S.Enteritidis (1 μg)
Dilution : 1:250

Anti-Salmonella enteritidis LPS antibody [se-1] used in Lateral Flow (Lateral Flow). GTX00861

GTX00861 Lateral Flow Image

Immunochromatography with GTX00861 Salmonella enteritidis LPS antibody [se-1]. Goat anti-S.Enteritidis serum and rabbit anti-Campylobacter jejuni serum were coated onto a specific upper area and under area, respectively, of the same nitrocellulose membrane, while goat anti-mouse IgG was coated onto another specific area (control line) on the same membrane. GTX00861 or C. jejuni MAb conjugated with colloidal gold were mixed with extract of each food poisoning bacteria in well. The strips were soaked and reacted with the mixture. Specific reactivity was shown in the well containing S. Enteritideis (left strip) and C. jujeni (right strip).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399