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Serine palmitoyltransferase 2 antibody [N1N3]

Anti-Serine palmitoyltransferase 2 antibody [N1N3] used in Western Blot (WB). GTX118192
Anti-Serine palmitoyltransferase 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX118192
Anti-Serine palmitoyltransferase 2 antibody [N1N3] used in Western Blot (WB). GTX118192

Cat. No. GTX118192

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Serine palmitoyltransferase 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

Mouse liver , IMR32

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Serine palmitoyltransferase 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731219

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine palmitoyltransferase long chain base subunit 2 , HSN1C , LCB2 , LCB2A , NSAN1C , SPT2 , hLCB2a

Cellular Localization

Endoplasmic reticulum membrane; Single-pass membrane protein

Background

This gene encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Serine palmitoyltransferase 2 antibody [N1N3] used in Western Blot (WB). GTX118192

GTX118192 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX118192 diluted at 1:1000

Anti-Serine palmitoyltransferase 2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX118192

GTX118192 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Serine palmitoyltransferase 2(GTX118192) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Serine palmitoyltransferase 2 antibody [N1N3] used in Western Blot (WB). GTX118192

GTX118192 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX118192 diluted at 1:500

REFERENCE

Application Reference

Kato M et al. Cosmetics 2017; 4 (3) : 33 Water-Soluble Organic Germanium Promotes Both Cornified Cell Envelope Formation and Ceramide Synthesis in Cultured Keratinocytes.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169