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Seryl-tRNA synthetase antibody [N1C2]

Anti-Seryl-tRNA synthetase antibody [N1C2] used in Immunoprecipitation (IP). GTX113546
Anti-Seryl-tRNA synthetase antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113546
Anti-Seryl-tRNA synthetase antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113546
Anti-Seryl-tRNA synthetase antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113546
Anti-Seryl-tRNA synthetase antibody [N1C2] used in Western Blot (WB). GTX113546

Cat. No. GTX113546

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Seryl-tRNA synthetase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Seryl-tRNA synthetase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037938

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

seryl-tRNA synthetase , NEDMAS , SERRS , SERS

Cellular Localization

Cytoplasm

Background

This gene belongs to the class II amino-acyl tRNA family. The encoded enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Seryl-tRNA synthetase antibody [N1C2] used in Immunoprecipitation (IP). GTX113546

GTX113546 IP Image

Immunoprecipitation of Seryl-tRNA synthetase protein from 293T whole cell extracts using 5 μg of Seryl-tRNA synthetase antibody [N1C2] (GTX113546).
Western blot analysis was performed using Seryl-tRNA synthetase antibody [N1C2] (GTX113546).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Seryl-tRNA synthetase antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113546

GTX113546 IHC-P Image

Seryl-tRNA synthetase antibody [N1C2] detects Seryl-tRNA synthetase protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Seryl-tRNA synthetase antibody [N1C2] (GTX113546) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Seryl-tRNA synthetase antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113546

GTX113546 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Seryl-tRNA synthetase(GTX113546) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Seryl-tRNA synthetase antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113546

GTX113546 IHC-P Image

Seryl-tRNA synthetase antibody [N1C2] detects Seryl-tRNA synthetase protein at cytosol on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
Seryl-tRNA synthetase antibody [N1C2] (GTX113546) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Seryl-tRNA synthetase antibody [N1C2] used in Western Blot (WB). GTX113546

GTX113546 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Seryl-tRNA synthetase antibody [N1C2] (GTX113546) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169