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Siglec11 antibody

Anti-Siglec11 antibody used in IHC (Paraffin sections) (IHC-P). GTX85285
Anti-Siglec11 antibody used in IHC (Paraffin sections) (IHC-P). GTX85285
Anti-Siglec11 antibody used in Western Blot (WB). GTX85285

Cat. No. GTX85285

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all SIGLEC11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

HepG2 Cell Lysate(GTX27900) , Human Liver Tissue Slide

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Siglec11 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human Siglec11.The immunogen is located within the last 50 amino acids of Siglec11.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sialic acid binding Ig like lectin 11

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Siglecs are sialic acid-binding lectins of the immunoglobulin superfamily that are mainly expressed in cells of the hematopoietic system. Siglec11 is unlike other siglecs in that it binds specifically to a2-8-linked sialic acids and is not found in peripheral blood leukocytes but instead on macrophages of various tissues. Siglec11 is highly homologous to Siglec10 over their extracellular domain but not over their cytoplasmic domain, suggesting that Siglec11 arose through gene duplication followed by a recombination event involving another ancestral siglec gene. Following treatment of Siglec11-transfected cells with pervanadate, Siglec11 becomes tyrosine-phosphorylated and strongly associates with SHP-1 and SHP-2. At least five isoforms of Siglec11 are known to exist. Siglec11 antibody will not cross-react with Siglec10. Despite its predicted size, Siglec11 migrates at a higher than expected molecular weight in SDS-PAGE.

Database

Research Area

DATA IMAGES

Anti-Siglec11 antibody used in IHC (Paraffin sections) (IHC-P). GTX85285

GTX85285 IHC-P Image

IHC-P analysis of human liver tissue using GTX85285 Siglec11 antibody.
Working concentration : 5 μg/ml

Anti-Siglec11 antibody used in IHC (Paraffin sections) (IHC-P). GTX85285

GTX85285 IHC-P Image

IHC-P analysis of human liver tissue using GTX85285 Siglec11 antibody.
Working concentration : 20 μg/ml

Anti-Siglec11 antibody used in Western Blot (WB). GTX85285

GTX85285 WB Image

WB analysis of HepG2 cell lysate using GTX85285 Siglec11 antibody.
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399