Sodium/Potassium ATPase beta 1 antibody

Cat. No. GTX113390

Reference 12

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Sodium/Potassium ATPase beta 1 products

View product citations for antibody GTX113390 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Rat brain , mouse brain

Predict Reactivity

Rabbit, Sheep, Dog, Guinea pig, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Sodium Potassium ATPase Beta 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036293

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase Na+/K+ transporting subunit beta 1 , ATP1B

Cellular Localization

Membrane

Background

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Gene ID: 481 ATP1B1
UniProt: P05026 ATP1B1

Research Area

DATA IMAGES

GTX113390 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase beta 1 antibody (GTX113390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113390 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase beta 1 antibody (GTX113390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113390 WB Image

Human tissue extract (5 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase beta 1 antibody (GTX113390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113390 IHC-P Image

Sodium Potassium ATPase Beta 1 antibody detects ATP1B1 protein at membrane on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
Sodium Potassium ATPase Beta 1 antibody (GTX113390) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113390 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using ATPase beta1(Na+/K+)(GTX113390) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113390 IHC-P Image

Sodium Potassium ATPase Beta 1 antibody detects ATP1B1 protein at membrane on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
Sodium Potassium ATPase Beta 1 antibody (GTX113390) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min