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Sorbitol Dehydrogenase antibody [2A10]

Overexpression
Anti-Sorbitol Dehydrogenase antibody [2A10] used in Western Blot (WB). GTX83583
Anti-Sorbitol Dehydrogenase antibody [2A10] used in Immunoprecipitation (IP). GTX83583
Anti-Sorbitol Dehydrogenase antibody [2A10] used in Western Blot (WB). GTX83583

Cat. No. GTX83583

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2A10

Isotype

 IgG2b

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($519)
See all Sorbitol Dehydrogenase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
IP 2ug/500ul
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human SORD expression vector

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sorbitol dehydrogenase , HEL-S-95n , SORD1

Background

Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.

Database

Research Area

DATA IMAGES

Anti-Sorbitol Dehydrogenase antibody [2A10] used in Western Blot (WB). GTX83583

GTX83583 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Sorbitol Dehydrogenase plasmid (Right) or empty vector (Left) for 48 hrs using GTX83583 Sorbitol Dehydrogenase antibody [2A10].
Loading : 5 ug per lane

Anti-Sorbitol Dehydrogenase antibody [2A10] used in Immunoprecipitation (IP). GTX83583

GTX83583 IP Image

IP analysis of DDDDK tagged Sorbitol Dehydrogenase overexpressed HEK293T lysate using GTX83583 Sorbitol Dehydrogenase antibody [2A10].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-Sorbitol Dehydrogenase antibody [2A10] used in Western Blot (WB). GTX83583

GTX83583 WB Image

WB analysis of various cell lines using GTX83583 Sorbitol Dehydrogenase antibody [2A10].
Loading : 35 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519