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Sox9a antibody

Anti-Sox9a antibody used in IHC (Whole mount) (IHC-Wm). GTX128370

Cat. No. GTX128370

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Sox9a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Predict Reactivity

Human, Mouse, Rat, Dog, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.12 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of zebrafish Sox9a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885765

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY (sex determining region Y)-box 9a , jef , wu:fj17b12 , zgc:111921

Database

Research Area

DATA IMAGES

Anti-Sox9a antibody used in IHC (Whole mount) (IHC-Wm). GTX128370

GTX128370 IHC-Wm Image

sox9a antibody detects sox9a protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
sox9a antibody (GTX128370) dilution: 1:200.

REFERENCE

Application Reference

Kraus JM et al. Development 2022; 149 (5) Notch signaling enhances bone regeneration in the zebrafish mandible.
Application : IHC-P
Reactivity : Zebrafish
Dash S et al. Development 2022; 149 (12) Nucleolin loss of function leads to aberrant Fibroblast Growth Factor signaling and craniofacial anomalies.
Moss JJ et al. FASEB J 2021; 35 (11) : e22002 Autophagy coordinates chondrocyte development and early joint formation in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Schulz A et al. J Histochem Cytochem 2019; 67 (5) : 361-373 Non-pathological Chondrogenic Features of Valve Interstitial Cells in Normal Adult Zebrafish.
Paul S et al. Development 2016; (Epub) Ihha induces hybrid cartilage-bone cells during zebrafish jawbone regeneration.
Application : IHC-P
Reactivity : Zebrafish
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169