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Splicing factor 1 antibody [N1N3]

KO/KD
Anti-Splicing factor 1 antibody [N1N3] used in Western Blot (WB). GTX104540
Anti-Splicing factor 1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104540

Cat. No. GTX104540

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Splicing factor 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Raji

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Mouse, Rat, Zebrafish, Rabbit, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human splicing factor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951880

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

splicing factor 1 , BBP , D11S636 , MBBP , ZCCHC25 , ZFM1 , ZNF162

Cellular Localization

Nucleus

Background

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Splicing factor 1 antibody [N1N3] used in Western Blot (WB). GTX104540

GTX104540 WB Image

Sample(30 ug whole cell lysate)
A:Raji (GTX27908)
7.5% SDS PAGE
GTX104540 diluted at 1:1000

Anti-Splicing factor 1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104540

GTX104540 ICC/IF Image

splicing factor 1 antibody [N1N3] detects splicing factor 1 protein at nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: splicing factor 1 protein stained by splicing factor 1 antibody [N1N3] (GTX104540) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB, ICC/IF, IP
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169