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Stanniocalcin 2 antibody

Anti-Stanniocalcin 2 antibody used in Western Blot (WB). GTX115113
Anti-Stanniocalcin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115113
Anti-Stanniocalcin 2 antibody used in Western Blot (WB). GTX115113

Cat. No. GTX115113

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Stanniocalcin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

A549 , H1299 , MCF-7 , Mouse kidney

Predict Reactivity

Rat, Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Stanniocalcin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885353

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stanniocalcin 2 , STC-2 , STCRP

Cellular Localization

Secreted

Background

This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Stanniocalcin 2 antibody used in Western Blot (WB). GTX115113

GTX115113 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: MCF-7
12% SDS PAGE
GTX115113 diluted at 1:1000

Anti-Stanniocalcin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115113

GTX115113 ICC/IF Image

Stanniocalcin 2 antibody detects Stanniocalcin 2 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Stanniocalcin 2 protein stained by Stanniocalcin 2 antibody (GTX115113) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Stanniocalcin 2 antibody used in Western Blot (WB). GTX115113

GTX115113 WB Image

Stanniocalcin 2 antibody detects Stanniocalcin 2 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Stanniocalcin 2 antibody (GTX115113) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169