Survivin antibody [60.11]

Cat. No. GTX24470

Host	Mouse
Clonality	Monoclonal
Clone Name	60.11
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μl ($429)

See all Survivin products

Datasheet
Document

View product citations for antibody GTX24470 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000
ICC/IF	1:50 - 1:200
IHC-P	1:50 - 1:200
IHC-Fr	Assay dependent
FACS	1 μg/ml
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Product Note

This antibody is specific for the cytoplasmic form of Survivin.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant human Survivin [UniProt# O15392]

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

baculoviral IAP repeat containing 5 , API4 , EPR-1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq]

Database

Gene ID: 332 BIRC5
UniProt: O15392 BIRC5

Research Area

DATA IMAGES

GTX24470 WB Image

WB analysis of mock or overexpressing Survivin cell lysate using GTX24470 Survivin antibody [60.11].
Loading : 5μg

GTX24470 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX24470 Survivin antibody [60.11].
Green : primary antibody
Blue : Hoechst 33258

GTX24470 IHC-P Image

IHC-P analysis of human ovary cancer tissue using GTX24470 Survivin antibody [60.11].

GTX24470 FACS Image

FACS (Intracellular staining) analysis of U2OS cells using GTX24470 Survivin antibody [60.11].
Blue : Primary antibody
Orange : Isotype control
Dilution : 5 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

GTX24470 FACS Image

FACS (Intracellular staining) analysis of A549 cells using GTX24470 Survivin antibody [60.11].
Blue : Primary antibody
Orange : Isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

GTX24470 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX24470 Survivin antibody [60.11].
Green : primary antibody
Blue : Hoechst 33258

GTX24470 WB Image

WB analysis of HeLa cell lysate (30μg) using GTX24470 Survivin antibody [60.11].
Loading : 30μg
Dilution : 1 μg/ml

GTX24470 FACS Image

FACS (Intracellular staining) analysis of Daudi cells using GTX24470 Survivin antibody [60.11].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 429

Qty

Survivin antibody [60.11]

Cat. No. GTX24470

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX24470 WB Image

GTX24470 ICC/IF Image

GTX24470 IHC-P Image

GTX24470 FACS Image

GTX24470 FACS Image

GTX24470 ICC/IF Image

GTX24470 WB Image

GTX24470 FACS Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.