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Swi6 antibody

Anti-Swi6 antibody used in Western Blot (WB). GTX48740
Anti-Swi6 antibody used in Western Blot (WB). GTX48740

Cat. No. GTX48740

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Schizosaccharomyces pombe
Package
50 μg ($399)
See all Swi6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ELISA 1:5000-1:30000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Schizosaccharomyces pombe

Immunogen

Synthetic peptide corresponding aa 314-328 of S.pombe Swi6 protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SPAC824.10c

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-Swi6 antibody used in Western Blot (WB). GTX48740

GTX48740 WB Image

WB analysis of Schizosaccharomyces pombe lysate using GTX48740 Swi6 antibody.
Loading : 35 μg
Dilution : 1:700

Anti-Swi6 antibody used in Western Blot (WB). GTX48740

GTX48740 WB Image

Western blot analysis is shown using GeneTex's Affinity Purified anti-Swi6 antibody to detect endogenous protein present in S.pombe lysate (arrowhead). Comparison to a molecular weight marker (not shown) indicates a band of ~43 kDa corresponding to S.pombe Swi6 protein. ~35ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 mm nitrocellulose. The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399