GeneTex

  • Country / Location Selection

United States (US)

Synapsin II antibody [GT387]

Overexpression
Anti-Synapsin II antibody [GT387] used in Western Blot (WB). GTX635883
Anti-Synapsin II antibody [GT387] used in Western Blot (WB). GTX635883
Anti-Synapsin II antibody [GT387] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635883

Cat. No. GTX635883

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT387

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Synapsin II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Synapsin II. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909913

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synapsin II , SYNII

Cellular Localization

Cell junction, synapse

Background

This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family encodes a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal. Polymorphisms in this gene are associated with abnormal presynaptic function and related neuronal disorders, including autism, epilepsy, bipolar disorder and schizophrenia. Alternative splicing of this gene results in multiple transcript variants. The tissue inhibitor of metalloproteinase 4 gene is located within an intron of this gene and is transcribed in the opposite direction. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-Synapsin II antibody [GT387] used in Western Blot (WB). GTX635883

GTX635883 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody [GT387] (GTX635883) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Synapsin II antibody [GT387] used in Western Blot (WB). GTX635883

GTX635883 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody [GT387] (GTX635883) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Synapsin II antibody [GT387] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635883

GTX635883 ICC/IF Image

Synapsin II antibody [GT387] detects Synapsin II protein at synapse by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synapsin II stained by Synapsin II antibody [GT387] (GTX635883) diluted at 1:500.
Red: Tau, a axon marker, stained by Tau antibody (GTX130462) diluted at 1:500.

REFERENCE

There are currently no references for Synapsin II antibody [GT387] (GTX635883). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Synapsin II antibody [GT387] (GTX635883). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169