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Synapsin II antibody

Overexpression
Anti-Synapsin II antibody used in Western Blot (WB). GTX135273
Anti-Synapsin II antibody used in Western Blot (WB). GTX135273
Anti-Synapsin II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135273

Cat. No. GTX135273

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Synapsin II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Synapsin II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887469

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synapsin II , 2900074L19Rik , AI836018 , AI841723

Cellular Localization

Cell junction, synapse

Database

Research Area

DATA IMAGES

Anti-Synapsin II antibody used in Western Blot (WB). GTX135273

GTX135273 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (GTX135273) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synapsin II antibody used in Western Blot (WB). GTX135273

GTX135273 WB Image

Mouse tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (GTX135273) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 30718744

Anti-Synapsin II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135273

GTX135273 ICC/IF Image

Synapsin II antibody detects Synapsin II protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synapsin II stained by Synapsin II antibody (GTX135273) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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Package List Price ($)
$ 399
$ 169