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Synapsin II antibody

Overexpression
Anti-Synapsin II antibody used in Western Blot (WB). GTX135310
Anti-Synapsin II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135310
Anti-Synapsin II antibody used in Western Blot (WB). GTX135310
Anti-Synapsin II antibody used in IHC (Paraffin sections) (IHC-P). GTX135310
Anti-Synapsin II antibody used in IHC (Paraffin sections) (IHC-P). GTX135310

Cat. No. GTX135310

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Synapsin II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:500000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Product Note

This antibody was raised against Synapsin II, and it does not cross react with Synapsin I based on the internal testing.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse Synapsin II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2909842

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synapsin II , 2900074L19Rik , AI836018 , AI841723

Cellular Localization

Cell junction, synapse

Database

Research Area

DATA IMAGES

Anti-Synapsin II antibody used in Western Blot (WB). GTX135310

GTX135310 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (GTX135310) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synapsin II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135310

GTX135310 ICC/IF Image

Synapsin II antibody detects Synapsin II protein at Synapse by immunofluorescent analysis.
Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synapsin II stained by Synapsin II antibody (GTX135310) diluted at 1:500.
Red: Tau, a marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

Anti-Synapsin II antibody used in Western Blot (WB). GTX135310

GTX135310 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (GTX135310) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Synapsin II antibody used in IHC (Paraffin sections) (IHC-P). GTX135310

GTX135310 IHC-P Image

Synapsin II antibody detects Synapsin II protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Synapsin II stained by Synapsin II antibody (GTX135310) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synapsin II antibody used in IHC (Paraffin sections) (IHC-P). GTX135310

GTX135310 IHC-P Image

Synapsin II antibody detects Synapsin II protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Synapsin II stained by Synapsin II antibody (GTX135310) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Package List Price ($)
$ 399
$ 169