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Synaptotagmin 1 antibody, Internal

Anti-Synaptotagmin 1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88461
Anti-Synaptotagmin 1 antibody, Internal used in Western Blot (WB). GTX88461

Cat. No. GTX88461

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Synaptotagmin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P 4-6μg/ml

Note :

IHC-P
Human Brain shows vesicular staining in neuronal cell and axon.
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88461-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RDLQSAEKEEQEK, from the internal region of the protein sequence according to NP_005630.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptotagmin 1 , BAGOS , P65 , SVP65 , SYT

Cellular Localization

Cytoplasm

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Synaptotagmin 1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88461

GTX88461 IHC-P Image

IHC-P analysis of human brain using GTX88461 Synaptotagmin 1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

Anti-Synaptotagmin 1 antibody, Internal used in Western Blot (WB). GTX88461

GTX88461 WB Image

WB analysis of human hippocampus lysate using GTX88461 Synaptotagmin 1 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399