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Synaptotagmin 1 (phospho Thr202) + Synaptotagmin 2 (phospho Thr199) antibody

Orthogonal
Anti-Synaptotagmin 1 (phospho Thr202) + Synaptotagmin 2 (phospho Thr199) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50250

Cat. No. GTX50250

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all Synaptotagmin 1 + Synaptotagmin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Product Note

The sequence around Synaptotagmin 1 (phospho Thr202) is conserved with the sequence around Synaptotagmin 2 (phospho Thr199).

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 202/199 (R-K-T(p)-L-N) derived from human Synaptotagmin 2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptotagmin 2 , CMS7 , MYSPC , SytII

Background

This gene encodes a synaptic vesicle membrane protein. The encoded protein is thought to function as a calcium sensor in vesicular trafficking and exocytosis. Mutations in this gene are associated with myasthenic syndrome, presynaptic, congenital, with or without motor neuropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-Synaptotagmin 1 (phospho Thr202) + Synaptotagmin 2 (phospho Thr199) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50250

GTX50250 ICC/IF Image

Orthogonal

ICC/IF analysis of methanol-fixed HeLa cells using GTX50250 Synaptotagmin 1 (phospho Thr202) + Synaptotagmin 2 (phospho Thr199) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399