GeneTex

  • Country / Location Selection

United States (US)

Syntaphilin antibody

Anti-Syntaphilin antibody used in Western Blot (WB). GTX85393
Anti-Syntaphilin antibody used in IHC (Paraffin sections) (IHC-P). GTX85393
Anti-Syntaphilin antibody used in IHC (Paraffin sections) (IHC-P). GTX85393

Cat. No. GTX85393

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Syntaphilin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Rat Brain Tissue Lysate(GTX27942)

Product Note

This antibody is specific for Syntaphilin Internal

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Syntaphilin antibody was raised against a 16 amino acid synthetic peptide from near the center of human Syntaphilin.The immunogen is located within amino acids 350 - 400 of Syntaphilin.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

syntaphilin

Cellular Localization

Membrane

Background

Syntaphilin was initially identified in a yeast two-hybrid screen with the carboxy terminal region of Syntaxin-1 as bait. Syntaxin-1 is a key component of the synaptic vesicle docking machinery that forms the SNARE complex with synaptobrevin and SNAP-25. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits the formation of the SNARE complex, thereby potentially regulating synaptic vesicle exocytosis. Syntaphilin also binds dynamin-1 and inhibits dynamin-dependent endocytosis. Mice lacking syntaphilin show an increased level of mitochondrial motility and a reduced density of axonal mitochondria. This correlates with an enhanced short-term facilitation and significant impairments in motor ability, suggesting syntaphilin plays a major role in presynaptic function. Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE. Multiple isoforms are known to exist.

Database

Research Area

DATA IMAGES

Anti-Syntaphilin antibody used in Western Blot (WB). GTX85393

GTX85393 WB Image

WB analysis of rat brain tissue lysate using GTX85393 Syntaphilin antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-Syntaphilin antibody used in IHC (Paraffin sections) (IHC-P). GTX85393

GTX85393 IHC-P Image

IHC-P analysis of human brain tissue using GTX85393 Syntaphilin antibody.
Working concentration : 5 μg/ml

Anti-Syntaphilin antibody used in IHC (Paraffin sections) (IHC-P). GTX85393

GTX85393 IHC-P Image

IHC-P analysis of human brain tissue using GTX85393 Syntaphilin antibody.
Working concentration : 20 μg/ml

REFERENCE

There are currently no references for Syntaphilin antibody (GTX85393). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Syntaphilin antibody (GTX85393). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399