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Syntenin 1 antibody [N1], N-term

Orthogonal
Anti-Syntenin 1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108391
Anti-Syntenin 1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108391
Anti-Syntenin 1 antibody [N1], N-term used in Immunoprecipitation (IP). GTX108391
Anti-Syntenin 1 antibody [N1], N-term used in Western Blot (WB). GTX108391
Anti-Syntenin 1 antibody [N1], N-term used in Western Blot (WB). GTX108391

Cat. No. GTX108391

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ELISA

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Syntenin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ELISA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Syntenin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951833

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

syndecan binding protein , MDA-9 , MDA9 , ST1 , SYCL , TACIP18

Cellular Localization

Cell junction , focal adhesion , adherens junction , Cell membrane; Peripheral membrane protein , Endoplasmic reticulum membrane; Peripheral membrane protein , Nucleus , Melanosome , Cytoplasm , cytosol , cytoskeleton

Background

The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Syntenin 1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108391

GTX108391 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Syntenin 1(GTX108391) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Syntenin 1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108391

GTX108391 IHC-P Image

Syntenin 1 antibody [N1], N-term detects SDCBP protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Syntenin 1 antibody [N1], N-term (GTX108391) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Syntenin 1 antibody [N1], N-term used in Immunoprecipitation (IP). GTX108391

GTX108391 IP Image

Immunoprecipitation of Syntenin 1 protein from U87-MG whole cell extracts using 5 μg of Syntenin 1 antibody [N1], N-term (GTX108391).
Western blot analysis was performed using Syntenin 1 antibody [N1], N-term (GTX108391).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Syntenin 1 antibody [N1], N-term used in Western Blot (WB). GTX108391

GTX108391 WB Image

Orthogonal

HeLa whole cell extracts and HeLa exosome extract (3.5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [N1], N-term (GTX108391) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Syntenin 1 antibody [N1], N-term used in Western Blot (WB). GTX108391

GTX108391 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [N1], N-term (GTX108391) diluted at 1:500.

REFERENCE

Application Reference

Chen JY et al. J Nutr Biochem 2013; 24 (11) : 1889-910 High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.
Application : ELISA
Reactivity : Human
Yu Y et al. Eur J Neurosci 2013; 38 (2) : 139038 Syntenin-a promotes spinal cord regeneration following injury in adult zebrafish.
Application : WB
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169