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TBR2 / Eomes antibody

Anti-TBR2 / Eomes antibody used in Western Blot (WB). GTX134088
Anti-TBR2 / Eomes antibody used in IHC (Paraffin sections) (IHC-P). GTX134088
Anti-TBR2 / Eomes antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134088
Anti-TBR2 / Eomes antibody used in IHC (Paraffin sections) (IHC-P). GTX134088

Cat No. GTX134088

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

mouse E10.5 embryo

Predict Reactivity

Human(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse TBR2 / Eomes. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

eomesodermin,C77258,TBR-2,Tbr2

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES
Anti-TBR2 / Eomes antibody used in Western Blot (WB). GTX134088

GTX134088 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TBR2 / Eomes antibody (GTX134088) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TBR2 / Eomes antibody used in IHC (Paraffin sections) (IHC-P). GTX134088

GTX134088 IHC-P Image

TBR2 / Eomes antibody detects TBR2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
TBR2 stained by TBR2 / Eomes antibody (GTX134088) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TBR2 / Eomes antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134088

GTX134088 ICC/IF Image

TBR2 / Eomes antibody detects TBR2 / Eomes protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TBR2 / Eomes stained by TBR2 / Eomes antibody (GTX134088) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TBR2 / Eomes antibody used in IHC (Paraffin sections) (IHC-P). GTX134088

GTX134088 IHC-P Image

TBR2 / Eomes antibody detects TBR2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
TBR2 stained by TBR2 / Eomes antibody (GTX134088) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf