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TBR2 / Eomes antibody

Anti-TBR2 / Eomes antibody used in Western Blot (WB). GTX134088
Anti-TBR2 / Eomes antibody used in IHC (Paraffin sections) (IHC-P). GTX134088
Anti-TBR2 / Eomes antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134088
Anti-TBR2 / Eomes antibody used in IHC (Paraffin sections) (IHC-P). GTX134088

Cat. No. GTX134088

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TBR2 / Eomes products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

mouse E10.5 embryo

Predict Reactivity

Human(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse TBR2 / Eomes. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887210

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eomesodermin , C77258 , TBR-2 , Tbr2

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-TBR2 / Eomes antibody used in Western Blot (WB). GTX134088

GTX134088 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TBR2 / Eomes antibody (GTX134088) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TBR2 / Eomes antibody used in IHC (Paraffin sections) (IHC-P). GTX134088

GTX134088 IHC-P Image

TBR2 / Eomes antibody detects TBR2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
TBR2 stained by TBR2 / Eomes antibody (GTX134088) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TBR2 / Eomes antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134088

GTX134088 ICC/IF Image

TBR2 / Eomes antibody detects TBR2 / Eomes protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TBR2 / Eomes stained by TBR2 / Eomes antibody (GTX134088) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TBR2 / Eomes antibody used in IHC (Paraffin sections) (IHC-P). GTX134088

GTX134088 IHC-P Image

TBR2 / Eomes antibody detects TBR2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
TBR2 stained by TBR2 / Eomes antibody (GTX134088) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169