APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , NCI-H929 , NCI-H929 nuclear extract
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human TCF3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885936
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
transcription factor 3 , AGM8 , E2A , E47 , ITF1 , TCF-3 , VDIR , bHLHb21 , p75
Cellular Localization
Nucleus
Background
The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX129237 WB Image
TCF3 / E2A antibody detects TCF3 protein by western blot analysis.
A. 30 μg Jurkat whole cell extract
B. 30 μg Raji whole cell extract
C. 30 μg NCI-H929 whole cell extract
7.5 % SDS-PAGE
TCF3 / E2A antibody (GTX129237) dilution: 1:2500
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GTX129237 WB Image
TCF3 / E2A antibody detects TCF3 protein by western blot analysis.
A. 30 μg NCI-H929 whole cell extract
B. 30 μg NCI-H929 nuclear extract
7.5 % SDS-PAGE
TCF3 / E2A antibody (GTX129237) dilution: 1:3000
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GTX129237 ICC/IF Image
TCF3 / E2A antibody detects TCF3 protein at nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TCF3 protein stained by TCF3 / E2A antibody (GTX129237) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX129237 IP Image
TCF3 / E2A antibody immunoprecipitates TCF3 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 50 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of TCF3 protein by 4 μg TCF3 / E2A antibody (GTX129237)
7.5 % SDS-PAGE
The immunoprecipitated TCF3 protein was detected by TCF3 / E2A antibody (GTX129237) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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REFERENCE
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REVIEW
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