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TCR V alpha 12.1 antibody [6D6.6]

Orthogonal
Anti-TCR V alpha 12.1 antibody [6D6.6] used in Flow cytometry (FACS). GTX79230
Anti-TCR V alpha 12.1 antibody [6D6.6] used in Western Blot (WB). GTX79230
Anti-TCR V alpha 12.1 antibody [6D6.6] used in Flow cytometry (FACS). GTX79230

Cat. No. GTX79230

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6D6.6

Isotype

 IgG1

Application

 WB, IHC-Fr, FACS, IP, Activation, IHC

Reactivity

 Human, Mouse, Rat
Package
200 μg ($399)
See all TCR V alpha 12.1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1-1:100
IHC-Fr Assay dependent
FACS 1-2 μg/test
IP Assay dependent
Activation Assay dependent
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human TCR V alpha 12.1

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_11162739

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

T cell receptor alpha locus , IMD7 , TCRA , TCRD , TRA@ , TRAC

Database

Research Area

DATA IMAGES

Anti-TCR V alpha 12.1 antibody [6D6.6] used in Flow cytometry (FACS). GTX79230

GTX79230 FACS Image

FACS analysis of PBMC cells using GTX79230 TCR V alpha 12.1 antibody [6D6.6] compared to an isotype control (blue).
Dilution : 2 ug/test incubated for 30 min at 4ºC

Anti-TCR V alpha 12.1 antibody [6D6.6] used in Western Blot (WB). GTX79230

GTX79230 WB Image

WB analysis of 25 ug of Jurkat (lane 1), CEM (lane 2) and rat thymus (lane 3) cell lysates using GTX79230 TCR V alpha 12.1 antibody [6D6.6].
Dilution : 1:10

Anti-TCR V alpha 12.1 antibody [6D6.6] used in Flow cytometry (FACS). GTX79230

GTX79230 FACS Image

Orthogonal

FACS analysis of positive PEER cells (left panel) or negative control Jurkat cells (right panel) using GTX79230 TCR V alpha 12.1 antibody [6D6.6].
Dilution : 5ul of primary antibody were used per test

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399