APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Note :
Depletion
in vivo γδ T cell depletion
|
Not tested in other applications.
Product Note
The UC3-10A6 monoclonal antibody reacts with an epitope on the delta chain of the mouse Vγ2 TCR (V gamma 2 T cell receptor).
PROPERTIES
Form
Liquid
Buffer
Filter-sterilized PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
G8 mouse T cells
Purification
Protein G purified
From tissue culture supernatant
Purity
>95% (Determined by SDS-PAGE)
Endotoxin
< 0.002 EU/弮g (Determined by LAL assay)
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
T cell receptor delta chain,Tcrd,Tcrdelta
Background
T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor alpha and delta loci. Both the alpha and delta loci include V (variable), J (joining), and C (constant) segments and the delta locus also includes diversity (D) segments. The delta locus is situated within the alpha locus, between the alpha variable and joining genes. During T cell development, the delta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The alpha chain is synthesized by recombination joining a single V segment with a J segment. For both chains, the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Ten variable segments can be used in either alpha or delta chains and are described by TRAV/DV symbols. Several V and J segments of the alpha locus are known to be incapable of encoding a protein and are considered pseudogenes. [provided by RefSeq, Aug 2012]
Database
REFERENCE
There are currently no references for TCR V gamma 2 antibody [UC3-10A6] (GTX14214). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TCR V gamma 2 antibody [UC3-10A6] (GTX14214). Be the first to share your experience with this product.